Friday, June 6, 2008
FUCKFUCKFUCKFUCKFUCK
LIFE STINKS. that's why even a clown is fucking depressed.
Sheesh. What the hell is going on here? Y am i always having reasons to be late??? Ytd i was late. Fuck...FUCKFUCKFUCKFUCKFUCK... also ytd's FYP session was a TOTAL let down
1. Our 100ml culture became a 47ml culture.
2. Our M15 pellet went missing because some smart alec of a TSO decided to throw it out or maybe it's just the stupid cleaner lady/ man.
IT'S NOT OVER YET.
3. Our culture should be induced with 0.1mM and 0.5mM of IPTG and instead all our cultures are NOW 0.1mM IPTG induced. FFFFFFFFFFFFFFFFFUCK...... WHY THE HELL IT HAPPENED? It is because we misread protocol and thought that it was the bacterial concentration instead of the IPTG concentration.
4. We forgot to change the nanometer for our spectrophotometer to OD405 and we still happily went back to consult Dr Ng after measuring all of them. -.- he embarassed us (in a way because he asked so loudly) why our OD was so low and he knew it.
FUCKKKKKKKKKKKKKKKKKKKKKKK..........
NOW I'M FUCKING EMO OSO...BECAUSE OUR PROTEIN IS NOT FUCKING BINDING TO AP, OR THERE'S NO FUCKING PROTEINS IN THE FIRST PLACE WHICH IS COMPLETELY IMPOSSIBLE BECAUSE IT'S FUCKING IMPOSSIBLE.
it's my new toothpick when i need it.
what did i do so wrong that you had to leave me alone ... at 10:52 AM
0 people in the crowd heard my words
0 people in the crowd heard my words
