Friday, July 11, 2008
"It's a gift, keep it"
Where Arwen gives the Evenstar to Aragorn in Lord of the Rings.
OOhhhhhhhh....I'm so tired!!! Slept for only 5 hours ytd and I have to wake up to go to school. Not something which I will be able to do like EVER again. That's why I HATEHATEHATE RP because of the school timings where we HAVE TO start school at 8:30pm or else our GPA will be down graded by 0.5!! GEEZ... WHAT NONSENSE. That's not the end of it, we have no absence allowed and the school does not accept medical certificates.
ICE CREAM THERAPY NEEDED!!!!!!!!!!!!!
My third meeting was the WORST today. I did not understand the "produce antibodies" and ended up screwing up badly in class and everyone was laffing!!!!!! LOL!!!!!! After that I continued with my slides but AGAIN, I screwed up with my words. I said "HP" instead of "pH" and the faci blames Su for distracting me!!! HELLO?????!! You're e one making me mess up my words dont blame Su! She's innocent!!! You're too strict!!!!!! CHANGE FACIIIIIIII.
Some of my frns and I went to the ladies (official gossip area) and starting *bytching abt our faci and we were toking abt e tym my other frn and i almost got busted! She was in e ladies too but luckily we dint let out any clue we were toking abt her. PHEW... just as we exited, we saw her a few steps away from us. OMGGGGGGGGGGGG, WE WERE ALL DEAD SILENT UNTIL WE WENT BACK TO CLASS. Then we all EXPLODED and everyone made a pact to not tok abt her as we'll never noe when she's gonna walk in or out a door. FREAKIN FUNNY.
Her RJ was like hard today but cuz ive done it b4......i was quite ok with it.
RJ:
A recombinant protein is also known as a chimera
protein. The fusion protein is produced by the E. coli
when a plasmid is being inserted into the host cell of
an E. coli before it is cultured to produce large scale.
There are different methods to purify the recombinant
proteins and one method which I found on the net was
using the Ni-NTA column and using different types of
buffers.
The column is then equilibrated with lysis buffer which
is attached a eppendorf tube. Then, the sample is poured
into the column using wash and elution buffer, it is
washed and eluted six times in total thrice for each
buffer. All flowthrough is collected and used in SDS
PAGE to review the results on which eluate has the most
purified protein in abundance.
Ni-NTA uses Metal- Chelate Affinity Chromatography which
is a newer way to purify the proteins. Affinity
purification of recombinant proteins is made easy using
an engineered six Histidine repeat at the N- or C-
terminus. This Histidine tag is capable of binding
nickel ions that are coupled to solid phase resins.
From here, it is able to produce more pure recombinant
proteins.
****************************
Went to meet my "HEY MAMAs" and FYP family after school and attended a small concert project. It was not well done even though there was good equipment. Probably it was all DIY whereas J's FYP did outsourcing which was a GREAT thing!!!!!!!! AHHAHA... i HEART my FYP family xcept u-noe-hu.
Where Arwen gives the Evenstar to Aragorn in Lord of the Rings.
OOhhhhhhhh....I'm so tired!!! Slept for only 5 hours ytd and I have to wake up to go to school. Not something which I will be able to do like EVER again. That's why I HATEHATEHATE RP because of the school timings where we HAVE TO start school at 8:30pm or else our GPA will be down graded by 0.5!! GEEZ... WHAT NONSENSE. That's not the end of it, we have no absence allowed and the school does not accept medical certificates.
ICE CREAM THERAPY NEEDED!!!!!!!!!!!!!
My third meeting was the WORST today. I did not understand the "produce antibodies" and ended up screwing up badly in class and everyone was laffing!!!!!! LOL!!!!!! After that I continued with my slides but AGAIN, I screwed up with my words. I said "HP" instead of "pH" and the faci blames Su for distracting me!!! HELLO?????!! You're e one making me mess up my words dont blame Su! She's innocent!!! You're too strict!!!!!! CHANGE FACIIIIIIII.
Some of my frns and I went to the ladies (official gossip area) and starting *bytching abt our faci and we were toking abt e tym my other frn and i almost got busted! She was in e ladies too but luckily we dint let out any clue we were toking abt her. PHEW... just as we exited, we saw her a few steps away from us. OMGGGGGGGGGGGG, WE WERE ALL DEAD SILENT UNTIL WE WENT BACK TO CLASS. Then we all EXPLODED and everyone made a pact to not tok abt her as we'll never noe when she's gonna walk in or out a door. FREAKIN FUNNY.
Her RJ was like hard today but cuz ive done it b4......i was quite ok with it.
RJ:
A recombinant protein is also known as a chimera
protein. The fusion protein is produced by the E. coli
when a plasmid is being inserted into the host cell of
an E. coli before it is cultured to produce large scale.
There are different methods to purify the recombinant
proteins and one method which I found on the net was
using the Ni-NTA column and using different types of
buffers.
The column is then equilibrated with lysis buffer which
is attached a eppendorf tube. Then, the sample is poured
into the column using wash and elution buffer, it is
washed and eluted six times in total thrice for each
buffer. All flowthrough is collected and used in SDS
PAGE to review the results on which eluate has the most
purified protein in abundance.
Ni-NTA uses Metal- Chelate Affinity Chromatography which
is a newer way to purify the proteins. Affinity
purification of recombinant proteins is made easy using
an engineered six Histidine repeat at the N- or C-
terminus. This Histidine tag is capable of binding
nickel ions that are coupled to solid phase resins.
From here, it is able to produce more pure recombinant
proteins.
****************************
Went to meet my "HEY MAMAs" and FYP family after school and attended a small concert project. It was not well done even though there was good equipment. Probably it was all DIY whereas J's FYP did outsourcing which was a GREAT thing!!!!!!!! AHHAHA... i HEART my FYP family xcept u-noe-hu.
what did i do so wrong that you had to leave me alone ... at 11:17 AM
0 people in the crowd heard my words
0 people in the crowd heard my words
