Thursday, July 10, 2008
It was raining today.
"Rain, rain, go away..."
Children were singing.
Solemn drops of tears from the high heavens.
Angels weeping, ah!
Perhaps from joy, laughter and excitement.
There is love in the air.
And maybe a little sweet someone in my heart.
Had the worst night ever, hardly slept at all. So heartbroken!!! =((( Washed up and started out for school. DARN IT, I din't get the early bus and it was PACKED. I had to stand for 1/2 the journey and there was jam in the middle of the journey. RAWR* Met my dearest Xi er, "MUACKXZ..." ^^
Reached the lab(yea, I got FREAKING lab) today and turned on my com. James left an offline msg for me. SORRY JAMES!!!! I was toooooo tired to go online. Maybe tonight I might and we can chat!! ^^
The day was so-so today and I presented the questions in the worksheet.
OH NO!!!!!!!!! THE UT WAS SOOOOOOOOOOOO TOUGH!!! Upon opening the test questions, there was pure silence in the class for 10 minutes and I was O_O. Just gave up for a while and slouched in my chair, sipping at my only consolatione my steaming hot Horlicks. QQQ!!!!!!! I'M SO SCREWED.
NEWSFLASH!!!! 2ND DEATH BY UT.
OMG, JUST KILL ME NOW.
Going for my stupid training but, "YAY!!! Because I get to see my "Hey MAMAs" =)))
"Rain, rain, go away..."
Children were singing.
Solemn drops of tears from the high heavens.
Angels weeping, ah!
Perhaps from joy, laughter and excitement.
There is love in the air.
And maybe a little sweet someone in my heart.
Had the worst night ever, hardly slept at all. So heartbroken!!! =((( Washed up and started out for school. DARN IT, I din't get the early bus and it was PACKED. I had to stand for 1/2 the journey and there was jam in the middle of the journey. RAWR* Met my dearest Xi er, "MUACKXZ..." ^^
Reached the lab(yea, I got FREAKING lab) today and turned on my com. James left an offline msg for me. SORRY JAMES!!!! I was toooooo tired to go online. Maybe tonight I might and we can chat!! ^^
The day was so-so today and I presented the questions in the worksheet.
Qn1: Looking at the gel can you identify the transgenic sample? (The ladder used is a 100bp ladder ranging from 100bp~ 1200bp).
Ans:
-There is presence of excess primer because it did not bind to the transgenic genes.
-Both Samples A and B have the presence of 1. plant chloroplast and 2. soy lectin.
-Sample B has the presence of 1. 35 S Promoter and 2. NOS terminator
-Conclude that Sample B is the transgenic sample.
*Thnks to Yuki I was able to understand. Thanks man!
*Thnks to Yuki I was able to understand. Thanks man!
Qn: Explain the rationale behind the design of the experiment e.g. the order of addition of the reagents, the total volumes prepared, the choice of primers etc.
Ans:
-Higher Taq DNA polymerase concentration might give synthesis if non-specific products. If inhibitors are present, higher amount might be needed.
-Any inaccuracy in dNTPs increase the minsincorporation level.
-Such primers are used to prove that the sample is transgenic.
-PCR buffer is used to make sure the pH is maintained, supports the activity of the Taq polymerase and it dilutes the sample.
*******************OH NO!!!!!!!!! THE UT WAS SOOOOOOOOOOOO TOUGH!!! Upon opening the test questions, there was pure silence in the class for 10 minutes and I was O_O. Just gave up for a while and slouched in my chair, sipping at my only consolatione my steaming hot Horlicks. QQQ!!!!!!! I'M SO SCREWED.
NEWSFLASH!!!! 2ND DEATH BY UT.
OMG, JUST KILL ME NOW.
Going for my stupid training but, "YAY!!! Because I get to see my "Hey MAMAs" =)))
what did i do so wrong that you had to leave me alone ... at 3:31 PM
0 people in the crowd heard my words
0 people in the crowd heard my words
