Friday, July 4, 2008
Hey you, you down there.
(*muchlikefallin' deviant)
What type of faci gives a group discussion followed by a class discussion like ol pregnant lady? Oh that's right. Pregnant lady herself! I was reading on FRET and Y2H when J and K decided they'll talk about it in the discussion and in the end?!?! I HAVE NOTHING TO DISCUSS ABOUT!!! And then pregnant woman proceeds to say, " Beverly's the target today." !!!!!!!!!!!!!
-.- Fug la fug la~~ (thick Indian accent.)
In the end, I repeated J's Y2H (Yeast 2 Hybrid system) to her and I was made to do the summary for the team. Here's what I said.
"So, basically J's doing on the yeast two hybrid system and it consists of the bait and the prey. They are expressed as fusion proteins with a
transcriptional activating domain (AD, prey), and a DNA-binding domain (DBD, bait),
respectively. The bait and prey fusions are co-expressed where a physical interaction between the roteins leads to the reconstitution of a functional transcription factor that binds to the promoter
of a reporter gene. Consequently, transcription of the reporter gene is switched on. A
protein-protein interaction is therefore translated into reporter gene activity which can be
detected or measured.
K is doing in FRET where a donor and acceptor is bound to 2 proteins. The donor is then bombarded with waves that will cause it to emit a glow. If the acceptor is at most 10Nm to the acceptor, it will absorb the emission and will glow which signals protein protein interaction.
As for S, she will be doing on the comparision of BRET and FRET where BRET is better as it does not cause photo damage compared to FRET.
I have yet to prepare any new theories, however I might be working on Split Ubiqitin."
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! Hello!??!
I've yet to start and I've got 1hr and 15 mins more to understand and make a slide. SHOOT. I'm dead.
X_X
Ok I've made the slide and completed my presentation. Survived that. My team went last and did not have any questions from her, guess the pregnant woman's too tired to do anything. DUH. GREAT. It's the UT now. DEAD. X_X 1st time in the world. Death by UT.
Furthermore, my lappy took the longest time EVER to set up UT agent and in the end i had to restart it like 7 mins before my UT. I was PANICKING like HELL. Luckily it everything started up 2 mins before my UT!! THANK YOU SO MUCH GOD!!! AMEN!
(*muchlikefallin' deviant)
What type of faci gives a group discussion followed by a class discussion like ol pregnant lady? Oh that's right. Pregnant lady herself! I was reading on FRET and Y2H when J and K decided they'll talk about it in the discussion and in the end?!?! I HAVE NOTHING TO DISCUSS ABOUT!!! And then pregnant woman proceeds to say, " Beverly's the target today." !!!!!!!!!!!!!
-.- Fug la fug la~~ (thick Indian accent.)
In the end, I repeated J's Y2H (Yeast 2 Hybrid system) to her and I was made to do the summary for the team. Here's what I said.
"So, basically J's doing on the yeast two hybrid system and it consists of the bait and the prey. They are expressed as fusion proteins with a
transcriptional activating domain (AD, prey), and a DNA-binding domain (DBD, bait),
respectively. The bait and prey fusions are co-expressed where a physical interaction between the roteins leads to the reconstitution of a functional transcription factor that binds to the promoter
of a reporter gene. Consequently, transcription of the reporter gene is switched on. A
protein-protein interaction is therefore translated into reporter gene activity which can be
detected or measured.
K is doing in FRET where a donor and acceptor is bound to 2 proteins. The donor is then bombarded with waves that will cause it to emit a glow. If the acceptor is at most 10Nm to the acceptor, it will absorb the emission and will glow which signals protein protein interaction.
As for S, she will be doing on the comparision of BRET and FRET where BRET is better as it does not cause photo damage compared to FRET.
I have yet to prepare any new theories, however I might be working on Split Ubiqitin."
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! Hello!??!
I've yet to start and I've got 1hr and 15 mins more to understand and make a slide. SHOOT. I'm dead.
X_X
Ok I've made the slide and completed my presentation. Survived that. My team went last and did not have any questions from her, guess the pregnant woman's too tired to do anything. DUH. GREAT. It's the UT now. DEAD. X_X 1st time in the world. Death by UT.
Furthermore, my lappy took the longest time EVER to set up UT agent and in the end i had to restart it like 7 mins before my UT. I was PANICKING like HELL. Luckily it everything started up 2 mins before my UT!! THANK YOU SO MUCH GOD!!! AMEN!
what did i do so wrong that you had to leave me alone ... at 4:00 PM
0 people in the crowd heard my words
0 people in the crowd heard my words
